1958-2014: After 56 Years of Research, Cytochrome P450 Reactivity Is Finally Explained
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چکیده
منابع مشابه
Cytochrome P450 Reactivity Is Finally Explained
The active sites of Cyt. P450s contain a single heme b cofactor with a proximal cysteinate ligand. Figure 1 shows the active site of P. putida Cyt. P450cam as an example. The catalytic mechanism of Cyt. P450s has been studied in detail and is depicted in Figure 1. After one-electron reduction of the heme, O2 is bound first and subsequently reduced and protonated, generating an Fe–OOH complex (C...
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Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}-5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerob...
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Since the publication of the first paper on "cytochrome P450" in 1962, the biochemical research on this novel hemoprotein expanded rapidly in the 1960s and the 1970s as its principal roles in various important metabolic processes including steroid hormone biosynthesis in the steroidogenic organs and drug metabolism in the liver were elucidated. Establishment of the purification procedures of mi...
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Genotype/phenotype analysis with human hepatocytes has identified a new inactive CYP2D6 allele, CYP2D6*56. Cryopreserved human hepatocytes from 51 livers were evaluated for CYP2D6 activity with dextromethorphan as the probe substrate. Hepatocyte lots that lacked CYP2D6 activity were further evaluated for CYP2D6 expression and known genetic variations, including CYP2D6*2, *3, *4, *5, *6, *7, *8,...
متن کاملShort Communication Cytochrome P450 2S1 is Reduced by NADPH-Cytochrome P450 Reductase
Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerobi...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2014
ISSN: 1433-7851
DOI: 10.1002/anie.201402404